CALCIFEDIOL decreases interleukin-6 secretion
Sept 10, 2019 0:02:46 GMT
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Calcifediol decreases interleukin-6 secretion by cultured human trophoblasts from GDM pregnancies
Marilyn Lacroix, Farah Lizotte, Marie-France Hivert, Pedro Geraldes, Patrice Perron Author Notes
Journal of the Endocrine Society, js.2019-00181, doi.org/10.1210/js.2019-00181
Published: 05 September 2019
Abstract
Gestational diabetes mellitus (GDM) is often characterized by low maternal calcifediol (25OHD) and high inflammation levels. This study aimed to determine if placental protein expressions of CYP27B1, VDR and CYP24A1 are impaired in GDM cases and to investigate the effect of a 25OHD treatment on IL-6 secretion by GDM trophoblasts compared to normoglycemic (NG) trophoblasts. Placental tissue samples were harvested to determine protein expression of CYP27B1, VDR and CYP24A1 by immunoblots. Isolated trophoblasts were stimulated with 25OHD concentrations (25-2000 nM) once a day for 3 days and IL-6 secretion was quantified (ELISA). We recruited 17 NG, 19 GDM women treated with diet and exercise alone (GDM-d) and 9 GDM women who necessitated insulin therapy (GDM-i). Protein expressions of CYP27B1 and VDR were significantly increased in placental tissue from GDM-d compared to NG (both p=0.02), while no differences were detected between GDM-i and NG placental tissues. In cultured trophoblasts (2 groups; n=5 NG and n=5 GDM-d), exposure to increasing 25OHD concentrations significantly decreased IL-6 secretion in the GDM-d group only (p=0.006). Following a treatment of 25OHD (2000 nM), IL-6 secretion was decreased in GDM-d compared to NG trophoblasts (p = 0.03). Our results suggest an upregulation of the VDR-1,25(OH)2D complex biodisponibility in GDM-d placentas, possibly reflecting a compensatory mechanism aiming to ensure that vitamin D can exert its genomic and non-genomic effects in the target cells of the placental-fetal unit. Our findings support an anti-inflammatory effect of vitamin D at the feto-maternal interface in GDM-d pregnancies.
Calcifediol decreases interleukin-6 secretion by cultured human trophoblasts from GDM pregnancies
Marilyn Lacroix, Farah Lizotte, Marie-France Hivert, Pedro Geraldes, Patrice Perron Author Notes
Journal of the Endocrine Society, js.2019-00181, doi.org/10.1210/js.2019-00181
Published: 05 September 2019
Abstract
Gestational diabetes mellitus (GDM) is often characterized by low maternal calcifediol (25OHD) and high inflammation levels. This study aimed to determine if placental protein expressions of CYP27B1, VDR and CYP24A1 are impaired in GDM cases and to investigate the effect of a 25OHD treatment on IL-6 secretion by GDM trophoblasts compared to normoglycemic (NG) trophoblasts. Placental tissue samples were harvested to determine protein expression of CYP27B1, VDR and CYP24A1 by immunoblots. Isolated trophoblasts were stimulated with 25OHD concentrations (25-2000 nM) once a day for 3 days and IL-6 secretion was quantified (ELISA). We recruited 17 NG, 19 GDM women treated with diet and exercise alone (GDM-d) and 9 GDM women who necessitated insulin therapy (GDM-i). Protein expressions of CYP27B1 and VDR were significantly increased in placental tissue from GDM-d compared to NG (both p=0.02), while no differences were detected between GDM-i and NG placental tissues. In cultured trophoblasts (2 groups; n=5 NG and n=5 GDM-d), exposure to increasing 25OHD concentrations significantly decreased IL-6 secretion in the GDM-d group only (p=0.006). Following a treatment of 25OHD (2000 nM), IL-6 secretion was decreased in GDM-d compared to NG trophoblasts (p = 0.03). Our results suggest an upregulation of the VDR-1,25(OH)2D complex biodisponibility in GDM-d placentas, possibly reflecting a compensatory mechanism aiming to ensure that vitamin D can exert its genomic and non-genomic effects in the target cells of the placental-fetal unit. Our findings support an anti-inflammatory effect of vitamin D at the feto-maternal interface in GDM-d pregnancies.